On the controversy of metal ion composition on amine oxygenase (AurF): a computational investigation.
نویسندگان
چکیده
An energetics-spectroscopic approach based on DFT method reveals that the active site structure of AurF has {Fe(III)(2)O} central core with one protonated terminal glutamate.
منابع مشابه
In vitro reconstitution and crystal structure of p-aminobenzoate N-oxygenase (AurF) involved in aureothin biosynthesis.
p-Aminobenzoate N-oxygenase (AurF) from Streptomyces thioluteus catalyzes the formation of unusual polyketide synthase starter unit p-nitrobenzoic acid (pNBA) from p-aminobenzoic acid (pABA) in the biosynthesis of antibiotic aureothin. AurF is a metalloenzyme, but its native enzymatic activity has not been demonstrated in vitro, and its catalytic mechanism is unclear. In addition, the nature of...
متن کاملA new class of arylamine oxygenases: evidence that p-aminobenzoate N-oxygenase (AurF) is a di-iron enzyme and further mechanistic studies.
Arylamine oxygenases are enzymes that catalyze the unusual oxidation of an aryl amine group to an aryl nitro group. Although arylamine oxygenases seem to be widespread and used in diverse metabolic reactions, 2] aminopyrrolnitrin oxygenase (PrnD) and p-aminobenzoate oxygenase (AurF) are the only two known examples. Recently, PrnD was biochemically and mechanistically characterized. AurF, howeve...
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The nonheme di-iron oxygenase, AurF, converts p-aminobenzoate (Ar-NH(2), where Ar = 4-carboxyphenyl) to p-nitrobenzoate (Ar-NO(2)) in the biosynthesis of the antibiotic, aureothin, by Streptomyces thioluteus. It has been reported that this net six-electron oxidation proceeds in three consecutive, two-electron steps, through p-hydroxylaminobenzoate (Ar-NHOH) and p-nitrosobenzoate (Ar-NO) interme...
متن کاملMetal-Ion-Coordinating Properties of Various Amino Acids, Investigation of the Essential Function in Biological Systems regarding to their Nano-Structure
The acidity constants of some amino acids (Am) were determined by potentiometric pH titration. The stability constants of the 1:1 complexes formed between M2+: Ca2+, Mg2+, Mn2+, Co2+, Ni2+, Cu2+ or Zn2+ and Am2-, were determined by potentiometric pH titration in aqueous solution (I = 0.1 M, NaNO3, 25°C). The order of the stability constants was reported. It is shown that the stability of the bi...
متن کاملMetal-Ion-Coordinating Properties of Various Amino Acids, Investigation of the Essential Function in Biological Systems regarding to their Nano-Structure
The acidity constants of some amino acids (Am) were determined by potentiometric pH titration. The stability constants of the 1:1 complexes formed between M2+: Ca2+, Mg2+, Mn2+, Co2+, Ni2+, Cu2+ or Zn2+ and Am2-, were determined by potentiometric pH titration in aqueous solution (I = 0.1 M, NaNO3, 25°C). The order of the stability constants was reported. It is shown that the stability of the bi...
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عنوان ژورنال:
- Physical chemistry chemical physics : PCCP
دوره 14 25 شماره
صفحات -
تاریخ انتشار 2012